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产品说明/Specification Sheet
产品名称: |
PHA-P (Phytohemagglutinin P) 植物血球凝聚集素P |
产品编号: |
L8754-5MG |
规格: |
5mg |
价格: |
¥180 |
产品类别: |
生化试剂 |
CAS#: |
9008-97-3 |
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品牌: |
Sigma |
级别: |
蛋白质 |
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分子式: |
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分子量: |
403.31 |
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危险品: |
NO |
危险品系数: |
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储存条件: |
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说明书: |
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产品属性:
Lectin from Phaseolus vulgaris (red kidney bean)
Phytohemagglutinin PHA-P, lyophilized powder
Description
Analysis Note |
Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures. |
Biochem/physiol Actions |
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins. |
Properties
form |
lyophilized powder |
potency |
<62.5 μg per mL agglutination activity |
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<10 μg/mL mitogenic activity |
total impurities |
salt, free |
storage temp. |
2-8°C |
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