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产品说明/Specification Sheet
产品名称: |
Pepsin 1:10000 胃蛋白酶1:10000 |
产品编号: |
P7000-25G |
规格: |
25g |
价格: |
¥480 |
产品类别: |
生化试剂 |
CAS#: |
9001-75-6 |
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品牌: |
Sigma |
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产品属性:
Description
Frequently Asked Questions |
Live Chat and Frequently Asked Questions are available for this Product. |
Analysis Note |
E1%/280=14.7 |
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Protein determined by E1%/280 |
Application |
Used to produce F(ab′)2 fragments of antibodies. |
Biochem/physiol Actions |
Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH>6. |
Other Notes |
View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer. |
Unit Definition |
One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16ml. Light path = 1cm.) |
Properties
form |
powder |
mol wt |
mol wt 35 kDa | |
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